previous method 33 data analysis Search Results


hmo11  (Elicityl Inc)
90
Elicityl Inc hmo11
Hmo11, supplied by Elicityl Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/hmo11/10__1039_slash_c7an01397c-83-97-61
Average 90 stars, based on 1 article reviews
hmo11 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
expert/symphony 1/1.5 t  (Siemens AG)
90
Siemens AG expert/symphony 1/1.5 t
Expert/Symphony 1/1.5 T, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/expert+symphony+1+1+5+t/pm40241075-152-261-287
Average 90 stars, based on 1 article reviews
expert/symphony 1/1.5 t - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
microinjection cannula components dummy cannula 33 gauge  (PlasticsOne inc)
90
PlasticsOne inc microinjection cannula components dummy cannula 33 gauge
Microinjection Cannula Components Dummy Cannula 33 Gauge, supplied by PlasticsOne inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/microinjection+cannula+components+dummy+cannula+33+gauge/pmc02474782-48-22-3
Average 90 stars, based on 1 article reviews
microinjection cannula components dummy cannula 33 gauge - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
hargreaves thermal stimulator model 33  (IITC Life Science)
90
IITC Life Science hargreaves thermal stimulator model 33
Hargreaves Thermal Stimulator Model 33, supplied by IITC Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/hargreaves+thermal+stimulator+model+33/pmc02483947-138-16-19
Average 90 stars, based on 1 article reviews
hargreaves thermal stimulator model 33 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
hpv-lbma  (MJ Research)
90
MJ Research hpv-lbma
Hpv Lbma, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/hpv+lbma/pmc01867512-122-33-24
Average 90 stars, based on 1 article reviews
hpv-lbma - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
γ 33 p-adenosine 5'-triphosphate  (GeneWorks)
90
GeneWorks γ 33 p-adenosine 5'-triphosphate
γ 33 P Adenosine 5' Triphosphate, supplied by GeneWorks, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/%CE%B3+33+p+adenosine+5++triphosphate/pmc01573471-57-11-15
Average 90 stars, based on 1 article reviews
γ 33 p-adenosine 5'-triphosphate - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
azo-initiators vazo 33  (DuPont de Nemours)
90
DuPont de Nemours azo-initiators vazo 33
Azo Initiators Vazo 33, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/2+2++azobis+4+methoxy+2+4+dimethylvaleronitrile++vazo+33/us10557018-67-5-33
Average 90 stars, based on 1 article reviews
azo-initiators vazo 33 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
p38 mapk  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology p38 mapk
FIGURE 4. HSP27 regulates IL-1-induced <t>p38</t> <t>MAPK</t> and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, <t>p38</t> <t>MAPK</t> was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.
P38 Mapk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/p38+MAP+Kinase+Inhibitor/10__1074_slash_jbc__m610987200-66-57-66
Average 93 stars, based on 1 article reviews
p38 mapk - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
antibiotics  (Thermo Fisher)
99
Thermo Fisher antibiotics
FIGURE 4. HSP27 regulates IL-1-induced <t>p38</t> <t>MAPK</t> and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, <t>p38</t> <t>MAPK</t> was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.
Antibiotics, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/Ampicillin/pm26869867-25-30-55
Average 99 stars, based on 1 article reviews
antibiotics - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
zeocin invitrogen  (Thermo Fisher)
99
Thermo Fisher zeocin invitrogen
FIGURE 4. HSP27 regulates IL-1-induced <t>p38</t> <t>MAPK</t> and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, <t>p38</t> <t>MAPK</t> was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.
Zeocin Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/Zeocin/pm24662969-62-6-7
Average 99 stars, based on 1 article reviews
zeocin invitrogen - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
refoil optimum 33 (o-33  (BASF)
90
BASF refoil optimum 33 (o-33
FIGURE 4. HSP27 regulates IL-1-induced <t>p38</t> <t>MAPK</t> and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, <t>p38</t> <t>MAPK</t> was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.
Refoil Optimum 33 (O 33, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/refoil+optimum+33++o+33/us09011692-170-36-41
Average 90 stars, based on 1 article reviews
refoil optimum 33 (o-33 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
kelzan s 3% solution in water  (Monsanto Technology LLC)
90
Monsanto Technology LLC kelzan s 3% solution in water
FIGURE 4. HSP27 regulates IL-1-induced <t>p38</t> <t>MAPK</t> and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, <t>p38</t> <t>MAPK</t> was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.
Kelzan S 3% Solution In Water, supplied by Monsanto Technology LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/previous+method+33+data+analysis/kelzan+s+3++solution+in+water/us08911851-120-9-16
Average 90 stars, based on 1 article reviews
kelzan s 3% solution in water - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


FIGURE 4. HSP27 regulates IL-1-induced p38 MAPK and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, p38 MAPK was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.

Journal: Journal of Biological Chemistry

Article Title: Heat Shock Protein 27 Functions in Inflammatory Gene Expression and Transforming Growth Factor-β-activated Kinase-1 (TAK1)-mediated Signaling

doi: 10.1074/jbc.m610987200

Figure Lengend Snippet: FIGURE 4. HSP27 regulates IL-1-induced p38 MAPK and JNK activity but not ERK activation. Cells were transfected as indicated and treated with IL-1 for different times. Cells were harvested and lysed. A, p38 MAPK was immunoprecipitated from lysates and assayed for its ability to phosphorylate His-tagged MK2. Graph shows mean p38 MAPK activity from three independent experiments (S.E.) as a percentage of peak activity in Scr-transfected cells. A portion of the lysates was Western-blotted for HSP27 and p38 MAPK. B, JNK was immu- noprecipitated from lysates, and JNK activity was assayed with GST-ATF-2 as a substrate. Graph shows JNK activity (as for A). An aliquot of lysate was analyzed by Western blotting for HSP27 and -tubulin. C, activation of ERK in lysates was detected by Western blotting with an antibody that recognizes ERK phos- phorylated on Thr-185 and Tyr-187. The blot was stripped and re-probed for total ERK. D, cells were transfected with Scr, HSP27#2, or HSP27#5 twice (with a 24-h interval between transfections) and harvested and lysed 24 h after the second transfection. Phosphorylation of p38 MAPK on Thr-180 and Tyr-182, JNK1 on Thr-183 and Tyr-185, JNK2 on Thr-221 and Tyr-223, and ERK (as in C) in lysates was examined by Western blotting with phosphospecific antibodies. p38 MAPK was blotted as a loading control. Data in each panel (A–D) are repre- sentative of at least two independent experiments.

Article Snippet: The membranes were stained with rabbit anti-COX-2 antibody (Alexis), a mouse anti- -tubulin antibody DM-1A (Sigma-Aldrich), a mouse monoclonal antibody against actin (Sigma), a rabbit antiserum raised against MKK3/6 (32), a rabbit antiserum to human HSP27 (7), a rabbit antiserum that recognizes both human HSP27 and murine HSP25 (Stressgen), a rabbit antiserum to the C-terminal peptide of p38 MAPK (33), a rabbit antiserum against TAK1 (sc-7162; Santa Cruz Biotechnology), and a rabbit antiserum against I B (sc-203; Santa Cruz Biotechnology).

Techniques: Activity Assay, Activation Assay, Transfection, Immunoprecipitation, Western Blot, Phospho-proteomics, Control

FIGURE 5. HSP27 is needed for activation by IL-1 of the p38 MAPK activators (MKK3 and MKK6) and the JNK activators (MKK4 and MKK7). Cells were transfected and treated as before. A, activation of MKK3 and MKK6 was detected with an antibody that recognizes phosphorylation of Ser-189 and Thr-193 of MKK3 and Ser-207 and Thr-211 of MKK6. The blot was stripped and re-probed with an antibody that recognizes total MKK3 and MKK6. B, kinase assay for MKK4 using a GST-JNK2 (K55R) (a mutant form that is incapable of auto- phosphorylation) and Western blots for HSP27 and -tubulin. C, kinase assay for MKK7 (as for B). Similar results were obtained in two separate experiments for each panel.

Journal: Journal of Biological Chemistry

Article Title: Heat Shock Protein 27 Functions in Inflammatory Gene Expression and Transforming Growth Factor-β-activated Kinase-1 (TAK1)-mediated Signaling

doi: 10.1074/jbc.m610987200

Figure Lengend Snippet: FIGURE 5. HSP27 is needed for activation by IL-1 of the p38 MAPK activators (MKK3 and MKK6) and the JNK activators (MKK4 and MKK7). Cells were transfected and treated as before. A, activation of MKK3 and MKK6 was detected with an antibody that recognizes phosphorylation of Ser-189 and Thr-193 of MKK3 and Ser-207 and Thr-211 of MKK6. The blot was stripped and re-probed with an antibody that recognizes total MKK3 and MKK6. B, kinase assay for MKK4 using a GST-JNK2 (K55R) (a mutant form that is incapable of auto- phosphorylation) and Western blots for HSP27 and -tubulin. C, kinase assay for MKK7 (as for B). Similar results were obtained in two separate experiments for each panel.

Article Snippet: The membranes were stained with rabbit anti-COX-2 antibody (Alexis), a mouse anti- -tubulin antibody DM-1A (Sigma-Aldrich), a mouse monoclonal antibody against actin (Sigma), a rabbit antiserum raised against MKK3/6 (32), a rabbit antiserum to human HSP27 (7), a rabbit antiserum that recognizes both human HSP27 and murine HSP25 (Stressgen), a rabbit antiserum to the C-terminal peptide of p38 MAPK (33), a rabbit antiserum against TAK1 (sc-7162; Santa Cruz Biotechnology), and a rabbit antiserum against I B (sc-203; Santa Cruz Biotechnology).

Techniques: Activation Assay, Transfection, Phospho-proteomics, Kinase Assay, Mutagenesis, Western Blot